Protein Production Strain Identification Tutorial

This tutorial provides an overview of a campaign, including a 3-tiered screening process and the importance of identifying the protein-producing strain. Practical exercises cover transformation, cultivation, and screening based on HiBiT tag, cPCR verification, and screening colonies for each construct. Specific step-by-step guides focus on secondary screening in fungal engineering, orthogonal validation of top isolates, and further testing and validation. Ideal for those involved in genetic ...